A Good Tim-1 Antibody from Thermo

January 23, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD365 (Tim-1) Monoclonal Antibody

Catalog Number:

12-3659-42

Clone Number:

1D12-TIM1

I used this antibody to stain mouse memory T cells (CD44+, CD62L+) ex vivo. Tim-1 is one of the exhaustion markers on T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well for staining Tim-1

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

This antibody works well for flow cytometry-based assays

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