A Good CD44 Antibody from Abcam

January 03, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Abcam

Product Name:

Recombinant Anti-CD44 antibody

Catalog Number:

ab51037

Clone Number:

EPR1013Y

I stained memory T cells using this antibody. CD44 is expressed on activated T cells and continues to express on memory T cells. The antibody works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

The antibody worked well and I could select the CD44-positive cells easily using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

This antibody works well in flow cytometry

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