A Good Enough PD1 Antibody from BioLegend

January 02, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD279 (PD-1) Antibody

Catalog Number:

135209

Clone Number:

29F.1A12

I used this antibody to probe for exhausted splenic T lymphocytes ex vivo. Exhausted CD8+ cells cease to function optimally by activating the expression of PD1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well but due to weak expression of PD1, there was a modest shift observed upon staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It is a good antibody.

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