A Good CD4 Antibody from BioLegend

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD4 Antibody

Catalog Number:

100510

Clone Number:

RM4-5

I stained mice Peripheral Blood Mononuclear cells fro flow cytometry based phenotyping. I used CD4 antibody for dump gating to select CD8+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Peripheral blood mononuclear cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

Cells were specifically stained using this antibody. There was no background after two washes with PBS.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No non-specific staining

The Bad

Nothing

The Bottom Line

Overall, its a great antibody for flow cytometry

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