A Good CD8a Antibody from BioLegend

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD8a Antibody

Catalog Number:

100705

Clone Number:

53-6.7

I performed phenotyping of transgenic mice and used this antibody to gate on CD8+ T lymphocytes. The antibody worked well and gave no background.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Peripheral blood monocytes cells

Primary Incubation

1:100

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

Cell population was distinctly stained and gating was easy to perform.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

Overall, a good product.

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