A Good VEGF Antibody for Western Blot

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

VEGF Antibody (A-20)

Catalog Number:

sc-152

Clone Number:

A-20

489 Inhaled Nitric Oxide After Oxidative Stress in Newborn Rats. Effects on Vegf Expression and Alveolarization were analyzed.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Exposure of newborn rats to hyperoxia decreases VEGF expression and impairs alveolarization. Much evidence indicates that NO plays a role in VEGF signaling and recent studies have shown that NO induces VEGF synthesis

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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