Santa Cruz Biotechnology, Inc.
Stat3 Antibody (C-20)
sc-8019
C-20
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Exogenous IL-10 at 10−8M inhibited PI cytokine release by 89–97%, while DEX at an equimolar level had no effect. DNA-binding activities of the PI transcription factors nuclear factor-κB (NF-κB) and activator protein-1 (AP-1), and the AI transcription factor signal transducer and activator of transcription 3 (STAT3) were induced over 18 h. DEX at 10−8M had no effect on any transcription factor DNA binding, but exogenous IL-10 at 10−8M produced a 60% inhibition of AP-1 DNA binding and enhanced phosphorylation of nuclear STAT3 for 18 h.
Western Blot
Cell lysate
1:1000 dilution in 5% skim milk prepared in 1x TBST
5% skim milk
1:4000 5% milk
None
ECL
Transcriptional Control of Cytokine Release from Monocytes of the Newborn: Effects of Endogenous and Exogenous Interleukin-10 versus Dexamethasone
N/A
No background and strong signal
Good antibody. Highly recommended.