A Good SP-C Antibody for Western Blot

January 17, 2020

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

SP-C Antibody (C-19)

Catalog Number:

sc-7705

Clone Number:

C-19

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Mutationally-activated PI3'-kinase-α promotes de-differentiation of lung tumors initiated by the BRAFV600E oncoprotein kinase.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody is highly specific and works great for western blot. Highly recommended.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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