Best DNA Biotin-Labeling Kit

Tissue DNA was quantified and employed to label with biotin at 3' end. For this, Pierce™ Biotin 3' End DNA Labeling Kit was used as per manufacturer instructions. In brief, DNA was mixed with a labelling reaction mixture and incubated for 30 minutes at 37°C. After incubation reaction was stopped with EDTA, chloroform: isoamyl alcohol added to each reaction mixture, centrifuged and the top aqueous layer was collected.DNA was precipitated from the aqueous layer and used for the downstream processing.