A Good SOD-2 Antibody for Western Blot

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

SOD-2

Catalog Number:

sc-133134.

Clone Number:

A-2

T oroxylin A induces p53 mitochondrial translocation and inhibits SOD2 activity. Additionally, our studies demonstrate that oroxylin A promotes the formation and mitochondrial translocation of the p53-Recql4 complex in HCT-116 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Curcumin enhanced manganese superoxide dismutase (MnSOD) protein expression inthe MCF-10F and Alpha3 cell lines.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

s. Curcumin enhanced manganese superoxide dismutase (MnSOD) protein expression.

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