A Good DUOX1 Antibody for Western Blot

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

Anti-DUOX1 antibody [N1N3]

Catalog Number:

GTX119160

Clone Number:

Polyclonal

The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1x TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:4000 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody is highly specific.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot. Highly recommended.

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