Santa Cruz Biotechnology, Inc.
HIF-1alpha (28b) Antibody
sc-13515
28b
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We cultured human mammary adenocarcinoma cell lines as spheroids with the hanging drop method and we observed a gradient of proliferating, quiescent, hypoxic, apoptotic and autophagic cells towards the inner core. This 3D organization decreased Trastuzumab sensitivity of HER2 over-expressing cells compared to monolayer cell cultures. We did not observe apoptosis induced by Trastuzumab but found cell arrest in G0/G1 phase. Moreover, the treatment downregulated the basal apoptosis only found in tumor spheroids, by eliciting protective autophagy.
Western Blot
Cell lysate
1:1000 dilution in 5% skim milk prepared in 1x TBST
5% skim milk
1:4000 5% milk
None
ECL
This antibody is highly specific and works great for western blot. Highly recommended.
N/A
No background and strong signal