Abcam
Anti-DMPO Nitrone Adduct antibody
ab23702
Polyclonal
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Pharmacological blockade of SLC6A14 causes amino acid starvation in estrogen receptor positive (ER+) breast cancer cells and suppresses their proliferation in vitro and in vivo. It transports all essential amino acids and the only excluded amino acids are glutamate and aspartate, which are nonessential. It also transports glutamine (an important precursor for nucleotide synthesis) and arginine (an amino acid essential for tumor growth). Here we checked the time course effect of our listed antibody by chemoattractant.
Western Blot
Tissue lysate
1:1000 dilution in 5% skim milk prepared in 1x TBST
5% skim milk
1:4000 5% milk
None
ECL
The primary DMPO nitrone adduct antibody (Abcam, ab23702). The appropriate HRP-conjugated secondary antibodies. Time course study performed.
N/A
No background and strong signal
Overall satisfactory