Staining CD44 in THP1 Cells

December 26, 2019

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Company:

Thermo Fisher Scientific

Product Name:

CD44 Monoclonal Antibody (IM7), APC

Catalog Number:

17-0441-82

Clone Number:

IM7

The aim is to detect CD44 on THP1 cells after PMA stimulation. It works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP1 cells

Primary Incubation

1/50 dilution

Blocking Agent

FC blocker

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Near 100% of THP1 cells expressed CD44 after PMA treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works well. No tricks.

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