Staining CD80 in THP1 Cells

December 17, 2019

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD80 Anti-human PE

Catalog Number:

130-110-371

Clone Number:

REA661

The aim was to detect CD80 on PMA activated THP1 cells. This antibody did its work.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP1 cells

Primary Incubation

1:11 for up to 10 cells/100 µL of buffer

Blocking Agent

FC blocker

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

There is a big portion of the cells stained positive for CD80 after PMA stimulation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works well.

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