Staining CD206 in THP1 Cells

December 19, 2019

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Company:

Thermo Fisher Scientific

Product Name:

CD206 (MMR) Monoclonal Antibody (19.2), eFluor 450

Catalog Number:

48-2069-41

Clone Number:

19.2

The aim was to detect CD206 on PMA activated THP1 cells. The antibody tested worked well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP1 cells

Primary Incubation

0.25 µg/million cells in 100ul buffer

Blocking Agent

FC blocker

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Big portion of the THP1 cells stained positive after PMA stimulation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use.

The Bad

None

The Bottom Line

It works.

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