Staining CD163 in THP1 Cells

December 23, 2019

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Company:

Thermo Fisher Scientific

Product Name:

CD163 Monoclonal Antibody PE-Cyanine7

Catalog Number:

25-1639-41

Clone Number:

eBioGHI/61 (GHI/61)

The aim was to detect CD163 on PMA-activated THP1 cells. The antibody tested worked.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human

Primary Incubation

0.5 µg/million cells in 100ul buffer

Blocking Agent

FC blocker

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Compared with unstained cells, there is a portion of the cells that stained positive for CD163.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It works.

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