Cell Signaling Technology
Phospho-AMPKα (Thr172)
2535
40H9
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Our lab has been interested in looking at how the AMPK signaling cascade affects downstream mTor, specifically phosphorylation at the Thr172 site therefore by activating tcells and then looking at western blot, we want to see whether there are changes in the level.
Western Blot
Activated murine CD8+ T cells
1:2000 overnight in 4C
5% nonfat dry milk
Anti-rabbit IgG HRP-linked
N/A
ECL Pico
The results we get from this antibody are reliable (we believe). The antibody shows one bright band consistently therefore we believe that the antibody works.
Reliable and good distinct bands
None
If the Thr172 site is where you are interested in looking at for western, I believe this antibody is perfect for you.