Very Nice Antibody for mTOR

January 03, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

mTOR (7C10) Rabbit mAb

Catalog Number:

2983

Clone Number:

7C10

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This antibody is great for both IF and WB.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymphocyte

Primary Incubation

1:300

Blocking Agent

10% goat serum

Secondary Incubation

Anti-rabbit AF488

Tertiary Incubation

None

Detection

Confocal microscope

Results Summary

Nice puncta staining for mTOR, indicating organelle localization (potentially lysosome).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very nice staining

The Bad

None

The Bottom Line

Great antibody for both IF and WB.

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