Good Ki-67 Staining

January 02, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Ki-67 Monoclonal Antibody (SolA15), PerCP-eFluor 710, eBioscience™

Catalog Number:

46-5698-82

Clone Number:

SolA15

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocyte

Primary Incubation

Surface staining

Blocking Agent

Fc Block

Secondary Incubation

1:2000 dilution

Tertiary Incubation

None

Detection

Flow Cytometer

Results Summary

Good staining but sometimes might be too bright.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

Need to titrate

The Bottom Line

Still good and specific staining. However, PerCP-eF710 might not be the best option for Ki-67.

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