Very Strong Detection of eIF4E

January 13, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Mouse Anti- eIF-4E

Catalog Number:

610269

Clone Number:

87/eIF-4E

Browse Similar Products: eIF4E Western Blot Antibodies

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This antibody gives a very strong signal with minimal nonspecific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit HRP

Tertiary Incubation

None

Detection

ECL detection

Results Summary

Very strong signal with minimal nonspecific bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific and strong

The Bad

None

The Bottom Line

Good antibody for western blot to detect eIF4E

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