Relatively Good Antibody for pAkt T308

January 10, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056

Catalog Number:

4056

Clone Number:

244F9

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This is a great antibody to look at the PI3K pathway.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit HRP

Tertiary Incubation

None

Detection

ECL detection

Results Summary

Relative good signal on T cells activated through TCR.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good detection of pAKT T308

The Bad

None

The Bottom Line

Great antibody to use to look at PI3K pathway.

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