Very Good Antibody for Phospho Akt

January 09, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060

Catalog Number:

4060

Clone Number:

D9E

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This antibody is very good.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit HRP

Tertiary Incubation

None

Detection

ECL detection

Results Summary

Resting mouse T cells have minimal p-Akt S473. Upon TCR stimulation, a very strong signal around the size of Akt appear. Very specific detection on the phosphorylation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

None

The Bottom Line

Very good antibody. Can be reused multiple times.

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