Very Good pS6 Staining

January 08, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #5044

Catalog Number:

5044

Clone Number:

D68F8

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This antibody gives distinct staining of pS6.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

FACS buffer

Blocking Agent

Fc block

Secondary Incubation

Surface staining then permeabilize

Tertiary Incubation

1:2000 RT 1hr

Detection

Flow cytometry

Results Summary

Very distinct staining of pS6 especially upon TCR activation on T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

None

The Bottom Line

Very useful tool to dissect mTORC1 activity of different cell type in a given tissue.

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