Good BioLegend Antibody for B220

January 06, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103241

Clone Number:

RA3-6B2

We are interested in studying the mechanism of lymphocyte differentiation through modulating mTOR pathway. This antibody gives very distinct staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

FACS buffer

Blocking Agent

Fc block

Secondary Incubation

1:500 4deg 20 min

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Very distinct staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining.

The Bad

None

The Bottom Line

Good antibody in relatively bright color

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