Relatively Good Antibody for CD44 Staining

January 03, 2020

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD44 Monoclonal Antibody (IM7), Alexa Fluor 700, eBioscience™

Catalog Number:

56-0441-82

Clone Number:

IM7

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway. This antibody gives good staining of CD44 in a dim channel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T cells

Primary Incubation

FACS buffer

Blocking Agent

Fc block

Secondary Incubation

1:400 4deg 20 min

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Very nice staining for CD44

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining on dim channel

The Bad

None

The Bottom Line

Good staining for CD44 in a dim channel

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