Good Antibody for Staining Vimentin Structure

December 23, 2019

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Rockland Immunochemicals

Product Name:

Vimentin Antibody Chicken Polyclonal

Catalog Number:

200-901-E54

Clone Number:

Polyclonal

We are interested in the dynamics of vimentin during T cell activation. This antibody is great

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse T cells

Primary Incubation

1:500

Blocking Agent

10% goat serum

Secondary Incubation

1:500

Tertiary Incubation

None

Detection

Anti-chicken Alexa Fluor 647

Results Summary

The antibody is great. The chicken host provides opportunity for multiplex imaging.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining.

The Bad

There are background non-specific staining in the knockouts.

The Bottom Line

Great for immunofluorescence for positive structural signal. Antibody still has some non-specific staining (not structural) in the knockout cells.

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