Permeabilization Buffer for Intracellular Transcription Factor Staining

Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

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Company:

Invitrogen

Product Name:

eBioscience™ Permeabilization Buffer (10X)

Catalog Number:

00-8333-56

This buffer came as 100ml of 10X solution. Need to dilute to 1X using water. This solution is used to permeabilize cells and stain intracellular transcription factors after fixation.

Experimental Design and Results Summary

Application

Perm/wash buffer for intracellular transcription factor staining

Starting Material

T cells

Tips

1 to 10 dilution in water

Results Summary

Easy to use for ICS staining, good results.

DOI or PMID #

N/A

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Summary

The Good

Easy to use

The Bad

Need to dilute

The Bottom Line

Commonly used to stain intracellular transcription factors.

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