Good Caspase-3 Antibody from CST

December 26, 2019

Biochemistry and Molecular Biology
University of Georgia
Other

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cleaved Caspase-3 (Asp175) Antibody

Catalog Number:

9661

We are working on delivering magnetic nanoparticles and anti-HIV drugs to the brain. We routinely use this antibody to identify cell undergoing apotosis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:200

Blocking Agent

Blocking buffer (bovine serum albumin, donkey serum and triton X100)

Secondary Incubation

1:400

Tertiary Incubation

2 hours

Detection

Immunofluorescence microscopy

Results Summary

Good stained images marker for cell undergoing apoptosis

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works really well

The Bad

N/A

The Bottom Line

Excellent antibody

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