BD Biosciences
BD Perm/Wash
554723
We use the BD Cytofix fixation to fix T cells stimulated with antigen and GolgiStop/GolgiPlug to stain intracellular cytokine staining. After washing out the fixation, we leave cells in this BD perm/wash buffer before pursuing the following ICS staining.
Perm/wash buffer to preserve cells before ICS staining
T cells
Need to do a 10 fold dilution in water
Easy to use.
N/A
Easy to use
Need to do dilutions
Easy and reliable to use for ICS cytokine staining.