BioLegend
Brilliant Violet 650™ anti-mouse CD8a Antibody
100742
53-6.7
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Browse Similar Products: CD8a Flow Cytometry Antibodies
We are an immunology lab that studies T cells. CD8+ T cells are a critical subset of cells in the immune system. We use flow cytometry experiments to identify and study CD8+ T cells in either in vitro or in vivo experiments. This antibody is very good to identify CD8 T cells since the BV650 results in distinct population as it's bright.
Flow Cytometry
Murine T cells from Spleen
1:500 in FACS buffer for 30 mins at 4 deg incubation
FC Block (CD16.32) in FACS solution during surface staining
N/A
Flow cytometer (Celesta)
We readily able to detect our donor CD8+ T cells along with the endogenous CD8+ T cells. As you can see, the signal is quite robust and separate from the negative cells.
Titrate appropriately since depends on experimental conditions.
Great
None
Indeed one of the better antibodies out there to identify CD8+ T cells using the BV650 color.