A Good vWF Antibody for IF Staining

December 09, 2019

BlueskyReddit
UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Von Willebrand factor antibody

Catalog Number:

ab11713

Clone Number:

Polyclonal

Tried to detect vWF in rabbit skin using anti-human vWF antibody. It worked well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rabbit skin

Primary Incubation

1/50 dilution, 45 min at RT

Blocking Agent

Cold aceton to fix for 10min, then 10% donkey serum to block

Secondary Incubation

Donkey anti sheep IgGAlexa fluor 488

Tertiary Incubation

1/1000 dilution, 45 min at RT

Detection

Fluorescence

Results Summary

This is an anti-human vWF antibody. it cross-reacts with mouse tissues. Here found that it cross-reacted with rabbit vWF too. The staining 100% overlapped with CD31 (not show).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Simple, easy to use.

The Bad

None

The Bottom Line

Just following our protocol, it works perfectly.

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