Great CD8 Antibody for Human

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711 anti-human CD8 Antibody

Catalog Number:

344733

Clone Number:

SK1

Our lab studies T cells throughout the whole human body and compares the differences across different organs. We need a reliable way to identify CD8 T cells so we can analyze them downstream. We chose this antibody because it is bright enough, but not too bright to leak into other colors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We are able to clearly stain the CD8+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody works well and reliably to stain CD8+ T cells in our human samples.

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