Robust Antibody to Analyze CD98 Expression in Cells

December 23, 2019

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Postdoctoral Fellow

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Company:

BioLegend

Product Name:

APC anti-mouse CD98 (4F2) Antibody

Catalog Number:

128212

Clone Number:

4F2

CD98 is an important subunit for amino acid transporters. Since mTOR controls the expression of this protein, we assess surface expression of CD98 on activated T cells using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Activated murine CD8 T cells

Primary Incubation

1:2000 in 2% FBS/PBS solution (FACS) at 4 deg for 30 minutes

Blocking Agent

FC Block 100ng/mL in FACS solution

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer Celesta

Results Summary

We can detect differential CD98 expression on T cells using flow cytometry.

DOI or PMID #

N/A

Additional Notes

Dilute antibody since it's quite bright.

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Summary

The Good

Great strong antibody

The Bad

None

The Bottom Line

Awesome antibody to detect CD98 surface expression using flow cytometry.

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