Great Multipurpose Antibody for c-MYC from CST

Oncology
Johns Hopkins School of Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

c-Myc (D84C12) Rabbit mAb

Catalog Number:

5605

Clone Number:

D84C12

Browse Similar Products: c-Myc Western Blot Antibodies

Our lab studies the role of metabolism in immune and tumor cells. c-MYC is a major transcription factor that regulates expression of metabolic enzymes. We use this specific antibody to analyze c-MYC expression levels in our samples via immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T cells

Primary Incubation

1:2000 in 4%BSA/TBST overnight at 4 deg

Blocking Agent

5% milk/TBST

Secondary Incubation

1:5000 anti-Rabbit HRP in 5%milk/TBST for 1 hour minimum

Tertiary Incubation

None

Detection

UVP

Results Summary

We can readily detect differential c-MYC levels in our lysates with western blotting.

DOI or PMID #

N/A

Additional Notes

Can reuse diluted antibody multiple times

Related Categories

Image Gallery

Summary

The Good

Minimal background and noise

The Bad

None

The Bottom Line

Great antibody to detect c-MYC in western lysates. Also can be used for other applications if needed, which is great.

Share your experience with other scientists. Write a Review! »

Join the discussion