Cell Signaling Technology
α-Tubulin (DM1A) Mouse mAb
3873
DM1A
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Browse Similar Products: a-Tubulin Western Blot Antibodies
We perform a lot western blots to look at signaling events but also expression of target proteins involved in T cell and macrophages. Proper loading is essential for all western blots, and we use routinely use tubulin as a loading control for equal protein assessment.
Western Blot
Tumor cells
1:2500 in 4% BSA/TBST overnight at 4 deg
5% milk in TBST RT for 1 hour
1:5000 anti-mouse HRP in 5% milk/TBST for 1 hour
None
UVP
We have equal protein loading in our experimental samples based on tubulin levels.
N/A
Robust antibody to detect tubulin but it can be used for microscopy as well
Great antibody for multiple applications especially western blots.