Lucigen
QuickExtract™ DNA Extraction Solution
QE09050
48 h post-CRIPSR-Cas9 Knock-in transfection, single cells were sorted into 96 well plates. Grown cell colonies were harvested and the cell pellet was resuspended in 20uL of Quick DNA extraction solution and kept at 37c for 20 min. 1ul of the sample was used to set up PCR reaction.
DNA extraction
Stem cells
I have extracted DNA from a minimum of 1000 cells and observed good PCR amplification.
1ul of DNA extracted by this method gave good amplification of the target gene with very less non-specific bands.
N/A
DNA can be extracted from very less cells
Easy method to extract quality DNA and best way to screen knock-in cells.