Cell Signaling Technology
Phospho-Akt (Thr308) (D25E6) mAB
13038
D25E6
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Our lab studies the role of cellular signaling in T cells during T cell differentiation and function. One important pathway of interest is the PI3K-AKT-mTOR pathway. With immunoblot analysis, we readily assessed p-AKT (T308) activity as a direct readout of upstream PI3K signaling in WT T cells upon T cell activation under different conditions.
Western Blot
Murine T cells with TCR activation
1:2000 in 4% BSA in TBST
5% dry milk in TBST
1:5000 Rabbit HRP in 5% dry milk in TBST
None
UVP imager
We can readily detect PI3K activity based on AKT activity at residue T308.
N/A
Reliable antibody
None whatsoever
Great antibody to detect Pi3K activity with western blotting.