Great VPS35 Antibody for Western Blots

December 04, 2019

Center for Neurodegenerative Science
Van Andel Institute
Lab Director / Manager

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Company:

Abcam

Product Name:

Anti-VPS35 antibody (ab57632)

Catalog Number:

ab57632

Clone Number:

Monoclonal

I use this antibody regularly for western blot detection of VPS35. This antibody reacts with mouse, rat, and human VPS35, although the abcam website only specifies human reactivity. Does not work well for immunofluorescence in rodent tissue.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK-293 cells, SH-SY5Y cells, rodent brain

Primary Incubation

1:1000 dilution incubated at 4 degrees overnight

Blocking Agent

5% non-fat dry milk

Secondary Incubation

1:10,000 with goat anti-mouse HRP antibody incubated for 1 hour at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Western blot analysis of mouse brain extracts showing VPS35 around 100 kDa.

DOI or PMID #

29893854

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable antibody for western blot detection of VPS35, very clean

The Bad

Does not work well for immunofluorescence

The Bottom Line

Great antibody for western blotting

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