Somehow This Buffer Does Not Work in My Hand

Seoul National University
Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

20X Bolt MOPS SDS Running Buffer

Catalog Number:

B0001

I used this for SDS-PAGE using NuPAGE Bis-tris gel. Somehow, it did not work out well.

Experimental Design and Results Summary

Application

SDS-PAGE of mammalian cell lysates

Starting Material

293T cells

Tips

I applied very low voltage but still the gels looked wavy.

Results Summary

Some optimization may be needed to make the gel run straight.

DOI or PMID #

N/A

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Summary

The Good

Not sure

The Bad

My gels looked wavy

The Bottom Line

I would use MES buffer instead of this...

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