Good DMPO Nitrone Adduct Western Blot Antibody from Abcam

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

Abcam

Product Name:

DMPO

Catalog Number:

ab23702

Clone Number:

None

Thioredoxin (Trx) is a 12kDa very small protein molecule known to be present in all organisms. It contains a dithiol-disulfide active site: -Cys-Gly-Pro-Cys-, and involved in many cell progress including cell growth, differentiation, and death. Here we checked the time course effect of DMPO by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of DMPO by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

Highly recommended...

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