Good NFKB Western Blot Antibody from Santa Cruz

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

NFkb P65

Catalog Number:

sc-372

Clone Number:

None

DCA inhibition of PDK frees up the mitochondrial gatekeeping enzyme PDH, which is then able to convert pyruvate to acetyl-CoA and initiate normal oxidative phosphorylation via the TCA cycle.Here we checked the time course effect of NFkb P65 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat Tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Nfkb p65 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion