Good Trx Western Blot Antibody from Santa Cruz

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

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Company:

Santa Cruz Biotech

Product Name:

Trx1

Catalog Number:

sc-20146

Clone Number:

Polyclonal

The thioredoxin (Trx) system is one of the central antioxidant systems in mammalian cells, maintaining a reducing environment by catalyzing electron flux from nicotinamide adenine dinucleotide phosphate through Trx reductase to Trx, which reduces its target proteins using highly conserved thiol groups. While the importance of protecting cells from the detrimental effects of reactive oxygen species is clear, decades of research in this field revealed that there is a network of redox-sensitive proteins forming redox-dependent signaling pathways that are crucial for fundamental cellular processes, including metabolism, proliferation, differentiation, migration, and apoptosis. Here we checked the time course effect of Trx by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat Tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Trx1 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

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