Santa Cruz Biotech
Trx1
sc-20146
Polyclonal
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The thioredoxin (Trx) system is one of the central antioxidant systems in mammalian cells, maintaining a reducing environment by catalyzing electron flux from nicotinamide adenine dinucleotide phosphate through Trx reductase to Trx, which reduces its target proteins using highly conserved thiol groups. While the importance of protecting cells from the detrimental effects of reactive oxygen species is clear, decades of research in this field revealed that there is a network of redox-sensitive proteins forming redox-dependent signaling pathways that are crucial for fundamental cellular processes, including metabolism, proliferation, differentiation, migration, and apoptosis. Here we checked the time course effect of Trx by chemoattractant.
Western Blot
Rat Tissue lysate samples
1:1000 dilution in 5% skim milk prepared in 1X TBST
5% skim milk prepared in 1X TBST
1:4000 5% skim milk prepared in 1X TBST
None
ECL
We found time dependent increase of Trx1 by chemoattractant.
N/A
No background and very strong signal
This antibody is very specific and the signal is strong. Highly recommended.