Good Antibody for Human CD4

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-human CD4 Antibody

Catalog Number:

344612

Clone Number:

SK3

Our lab focuses on T cells and their characterization in various human organ tissues. We frequently sort cells isolated from these organs and needed a bright and reliable CD4 cell marker for sorting, so we chose this one.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Influx

Results Summary

This antibody has good separation for identifying CD4+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This anitbody works well for us to identify and sort human CD4+ T cells.

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