BD Biosciences
Fixable Viability Stain 700
564997
An important part of flow cytometry analysis is being sure that we are analyzing only living cells for functional markers. Therefore, we like to use this bright viability marker to differentiate between live and dead cells before during further analysis.
Flow Cytometry
Human lung cells
Cells were stained in 100ul FACS buffer with antibody at 1:1000; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS
None
LSR II
This marker is absorbed by dead cells and live cells remain unstained.
N/A
Bright and stable
We are able to use this marker reliably to detect live mouse cells.