Good CD56 Antibody from BD Biosciences

School of Medicine
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BUV737 Mouse Anti-Human CD56

Catalog Number:

612766

Clone Number:

NCAM16.2

Browse Similar Products: CD56 Flow Cytometry Antibodies

I study human NK cells from blood or tissues (lymph node, certain types of solid tumors). CD56 is a positive marker for NK cells. I have used several CD56 antibodies, and found that this one is very good.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood (but also tissue)

Primary Incubation

1:100 dilution, incubated 30min at 4oC in the dark

Blocking Agent

None (don't needed in this settings)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry (FACSAria Fusion)

Results Summary

NK cells can be defined as CD45+Lineage(CD3/CD14/CD19/CD20)-CD56+ lymphocytes. Figure shows a dot plot of the expression of CD56 and CD16 on gated CD45+Lin- cells lymphocytes, from the peripheral blood. Using this gate, we can clearly see two subsets of NK cells: CD56bright (CD16+/-CD56bright) and CD56dim (CD16+CD56dim).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright color

The Bad

None

The Bottom Line

BUV737 Mouse Anti-Human CD56 antibody (clone NCAM16.2) from BD Biosciences is a good antibody to study human NK cells.

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