Direct PCR Lysis Reagent for Genotyping

December 02, 2019

BlueskyReddit
Drexel University
Pediatrics
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Viagen Biotech

Product Name:

DirectPCR Lysis Reagent (Mouse Tail)

Catalog Number:

102-T

This is a single-tube system for rapid preparation of DNA from several specimen including mouse tail. I have used for genotyping mice colony. The lysate from this reagent is good for genotyping but not suitable for sensitive PCR.

Experimental Design and Results Summary

Application

Genotyping

Starting Material

Mouse tail, Cells etc

Tips

Incubate for 50 min at 85°C for mouse tail. Can not use for specific PCR, because of inhibitors.

Results Summary

I have used this for genotyping and got the results. This is sensitive and time saving.

DOI or PMID #

N/A

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Summary

The Good

Very good, one step protocol.

The Bad

Can not use for specific PCR, because of inhibitors

The Bottom Line

This is a very good reagent, sensitive one step, time saving. Good for regular genotyping. Limitation is not suitable for specific PCR, because of inhibitors in it.

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