Sensitive Rabbit Monoclonal Antibody for Hsp70 Protein

November 26, 2019

Pediatrics
Drexel University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Novus Biological

Product Name:

HSP70/HSPA1A Antibody

Catalog Number:

NBP2-67287

Clone Number:

SA0379

I was looking to see the effect of molecular chaperone in hyperoxia in NRVC. Hsp70 is one of important player in macroautophagy. Hyperoxia increases Hsp70 expression and I got the results as expected.

Experimental Design and Results Summary

Applications

Western Blot

Sample

NRVC

Primary Incubation

1:1000, overnight on shaker in cold room

Blocking Agent

3%BSA

Secondary Incubation

1:5000, 1hr at room temp

Tertiary Incubation

N/A

Detection

LICOR IMAGE

Results Summary

My experiments went really well as shown in the figure. I have only used in W. blots

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good antibody for W. Blot

The Bad

None

The Bottom Line

The sensitivity and specificity of this is self explanatory by the provided image. I have only used in W. blots. I hope this antibody also work with other experiments.

Share your experience with other scientists. Write a Review! »

Join the discussion