A Good AF647 Rat Anti-Mouse Siglec-F from BD Biosciences

December 13, 2019

BlueskyReddit
Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 647 Rat Anti-Mouse Siglec-F

Catalog Number:

562680

Clone Number:

E50-2440

Browse Similar Products: Siglec-F Flow Cytometry Antibodies

I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse tissue single cell suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Efficient SiglecF expression detection on mouse immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody, strong signal and bright fluorophore

The Bad

None

The Bottom Line

Good antibody for efficient detection of SiglecF on mice immune cells.

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