A Good PE/Cy5 Anti-Mouse CD5 Antibody from BioLegend

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy5 anti-mouse CD5 Antibody

Catalog Number:

100610

Clone Number:

53-7.3

I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse tissue single cell suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Efficient CD5 expression detection on mouse immune cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody, strong signal and bright fluorophore

The Bad

Little background

The Bottom Line

Decent antibody for efficient detection of CD5 on mice immune cells.

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